CV of Technicienne de Laboratoire / Technicien superieur , looking for a job of Technicien superieur de laboratoire / laboratoires.enligne-se.com

Current industry: Nanobiotechnologies

Size of the company: 51-100 employees

Current position: Technicienne de Laboratoire

Number of years spent at this position: 1-2 years

Number of persons you managed: 0

Annual salary: 25000.00 EUR

Total working experience: 6-10 years

Availability: 3-months notice

Positions: Technicien superieur, Ingénieur technique, Assistant ingénieur

Industry: Industrie pharmaceutiques Recherche et développement, Institut de recherche,

Desired type of contract: open-ended contract, fixed term contract

Desired working time: Full-time, Part-time, Available on Week-ends

Minimum annual salary desired: 25000.00 / 30000.00 EUR

Highest educational level completed: number of years studied and completed with a diploma after highschool : +3

Last diploma : Licence biologie générale mention biologie cellulaire et physiologie.

Current educational level : +3

Other training courses :

Geographical mobility: Country : Belgium, Canada, Spain, United States, France, French Polynesia, Greece, Ireland, Italy, Liechtenstein, Luxembourg, Martinique, Monaco, Norway, Netherlands, Portugal, Sweden, Switzerland, United Kingdom

Known Tools / Software/ Methods Biologie moléculaire : Extraction et traitement d’ADN génomique, mitochondrial et bactérien, Southern-blot, PCR et analyse de microsatellites, PCR méthylsensible, PCR en temps réel, séquençage, construction de vecteur, transformation bactérienne. Biologie cellulaire : Entretien, congélation et décongélation de lignée cellulaire, transfection, sélection de clone cellulaire, étude de cytotoxicité et de résistance trans-épithéliale, étude de transport trans-épithélial. Biochimie : Technique, analyse et validation d’examen de biochimie de routine, Western-blot, dosage ELISA, analyse en fluorimétrie, électrophorèse bi-dimensionnelle, cytométrie de flux.

HGV, LGV, special vehicles licences Permis B

Languages French : Native
English : Working language
Spanish : Fluent
German : Basic
Basque : Basic

Miss Ma... F...

....

64700 Hendaye
France

skills

Molecular biology: Extraction and treatment of genomic, mitochondrial and bacterial DNA, Southern-blot, PCR and analysis of

microsatellites, methyl sensitive PCR, real time PCR, sequencing, vectorial design and construction, bacterial transformation.

Cellular biology: Cell lines maintenance, freezing and unfreezing, transfection, cell cloning experiments, toxicity assays (viability, genotoxicity, proteotoxicity, trans epithelial electrical resistance).

Biochemistry: Analysis and validation of biochemical assays, Western-blot, ELISA, flow cytometry, 2D protein electrophoresis.

Nanotechnology: Management, manipulation and characterization of nanostructures (Quantum dots).

Characterisation: Spectrometry, luminometry, fluometry, confocal microscopy Analysis.



Career

From May 2009: Nanobiotechnology Group, Pr. Nabiev, CIC NanoGUNE, San Sebastian-Spain. Permanent contract

• Technical referent of: Cell culture, flow cytometry, 2D protein electrophoresis, real time PCR.

• Technical follow-up of PhD Students, responsible of development of flow cytometry tests (setup, analysis et presentation of the results)

• Purchases, management of stocks and grants, installation and maintenance of laboratory equipments.

From June 2006 to May 2009: Laboratory of genetic diagnostic, service of Pr. Delpech, Cochin hospital, Paris. Permanent contract

• Diagnosis antenatal and postnatal of genetic diseases by Southern-blot, microsatellites analysis, real time PCR, methylsensitive PCR, and gene sequencing.

• Creation, setup, and validation of new methods of diagnostic, updating and improvement of existing methods.

• Training of technician personal to the real time PCR technology.

From June 2005 to May 2006: Laboratory of biochemistry and forensic appraisal, service of Pr de Mazancourt, Raymond Poincaré Hospital, Garches. Temporary contract

• Execution of routine biochemistry analysis, protein electrophoresis.

• Forensic analysis.

• Toxicological quantification.

From September 2001 to October 2003: Service of biochemistry, department of cardiovascular and thrombosis investigation and development, Sanofi-synthélabo, Chilly-Mazarin. Contract of apprenticeship

• Creation of a fluorimetry cellular test, bacterial cloning (plasmid construction, transformation, selection), cellular cloning (maintenance of cell culture, transfection, and clones selection).

• Western-blot, ELISA.



Education

University:

• October 2003-June 2004: Bachelor of biology mention cellular biology and physiology. Faculty of Créteil-ParisXII.

• Sept 2001-Sept 2003: DUT (technology universitary title) biological engineering apprenticeship curses, option of biological and biochemical analysis, in partnership with Sanofi-synthélabo. IUT (Technology University Institute) of Créteil-ParisXII.

• June 2001: Scientific high school diploma option life and earth sciences. High school Racine, Paris

Professional training:

• February 2010: Protein electrophoresis from A to Z. University of Strasbourg, Montpellier, France.

• January 2010: Training of laboratory safety, Professional training institute Don Bosco, Errenteria, Spain.



Computing skills

• Advanced user of Office applications (Word, Excel, PowerPoint).

• Advanced user of several biological analysis softwares.



Languages

• French: Mother tongue

• English: Read, written spoken fluently.

• Spanish: Read, spoken, fluently.

• German: Notions

Miss Ma... F...

....

64700 Hendaye
France

Emploi de technicien supérieur de laboratoire

Actuellement technicienne au sein d'un laboratoire pluridisciplinaire de nanotechnologies, je suis à la recherche d'un poste équivalent qui me permeterait de faire évoluer ma carrière.Toujours orientée vers l'innovation et les technologies de pointe, mon parcours professionnel m'a permis d'aborder des domaines tels que le recherche pharmaceutique, le diagnostic moléculaire de maladie génétique, l'expertise scientifique et judiciaire ou encore les nanobiotechnologies. Cet éventail d'expériences m'a permi d'acquérir les aptitudes et les compétences qui font d'un technicien experimenté un atout pour un laboratoire. Ma rigueur, ma polyvalence, et mon savoir-faire technique m'ont déjà permis de satisfaire aux exigences de projets ambitieux.Aujourd'hui, je souhaite investir mes acquis au sein d'une équipe dynamique et performante.

Miss Ma... F